Mr. Martin Falk Ph.D.
Future Forces Forum
Head of Department of Cell Biology and Radiobiology, Institute of Biophysics
The Czech Academy of Science
Detection of DSB Repair Foci as a Potent Tool in Biodosimetry and Cancer Research
Radiobiology currently experiences its renaissance, especially in light of the globally increased threat of terrorist attacks, potential industrial disasters, planned cosmic journeys, and (cancer) radiotherapy development. DNA double strand breaks (DSBs) represent the most serious DNA lesions induced by ionizing radiation and radiomimetic chemicals. In this lecture, we will focus on current possibilities of DSB detection and quantification by the means of H2AX immunofluorescence microscopy and flow cytometry. Applications of these techniques in biodosimetry and cancer research/medicine will be discussed. H2AX foci appear at sites of DSB in minutes after irradiation (as the consequence of H2AX histones phosphorylation on serine 139) and represent a specific, very sensitive DSB marker. The number of H2AX foci corresponds to that of DSBs and H2AX nuclear distribution is characteristic for high-LET and low-LET radiations.
2008: Special state exam: Radiation Protection Officer and Qualified Expert at Radiation Protection (workplaces with important sources of ionizing radiation)
2004: PhD. in Molecular Biology and Genetics, Faculty of Science, Masaryk University, Brno, Czech Republic
2003: DSc./M.A. (Doctor Rerum Naturalis; RNDr.) in Molecular Biology and Genetics, Faculty of Sciences, Masaryk University, Brno, CR.
2000: MSc. in Molecular Biology and Genetics, Faculty of Sciences, Masaryk University, Brno, CR.
1998: BSc. in Molecular Biology and Genetics, Faculty of Sciences, Masaryk University, Brno, CR.
Radiobiology, DNA double-strand break induction, repair and misrepair; mechanism of formation of chromosomal translocations, higher-order chromatin structure, function and dynamics in regulation of nuclear processes; carcinogenesis; leukemia development and pathogenesis; low/high-dose radiation risks and complex cellular response; tumor cell radiosensitization for radiotherapy; ion-beam cancer therapy, biological effects of different kinds of ionizing radiation
34 articles, 2 book chapters, cumulative IF = 97, citations > 483 (WOS), h-index = 11, > 30 invited lectures (including keynote and plenary lectures)
Awards: • (2015) Young Investigators Travel Award, 15th International Congress of Radiation Research (ICRR 2015), Kyoto, Japan •(2009) The Premium of Otto Wichterle for outstanding young scientists, devoted by the Academy of Sciences of CR •(2008) The Prize of Deutsche Gesellchaft für DNA-Reparaturforschung (DGDR), 10th Biennial Meeting of the DGDR, Berlin, Germany •(2004) The Prize of Institute of Biophysics of ASCR for excellent young scientists